Mouse brain
GSM9817885
mouse brain 705 MB 384,475 bins bronze H&E NCBI GEO policy
TechnologyVisium HD
Organismmouse
Tissuebrain
Bins384,475
bronze H&E NCBI GEO policy
Collections / Pericyte KATP Channel Hyperactivity Redistributes Cortical Blood Flow in a CADASIL Mouse Model
Pericyte KATP Channel Hyperactivity Redistributes Cortical Blood Flow in a CADASIL Mouse Model
Spatial transcriptomics on WT versus CADASIL mouse model with brain and isolated vasculature
Datasets8
Size~3.3 GB
AssaysVisium HD · Visium
Samplemouse · multiple
KindComparison
Download the whole collection
Python · sddb client
from sddb import Catalog
cohort = Catalog().collection("geo-gse335702")
cohort.members # the 8 datasets as a table
print(cohort.licenses()) # check the terms before you download
sdatas = cohort.to_dict() # {uid: SpatialData}, cached locally
# or keep the .zarr files in a folder of your choice:
# sdatas = cohort.to_dict(dest="data/") Python · lamindb
Needs pip install lamindb and network access to the Lamin database, which is limited during the alpha. If it fails, use the Client tab.
import lamindb as ln
db = ln.DB("scverse/spatialdata-db")
coll = db.Collection.get(key="geo-gse335702")
coll.ordered_artifacts.to_dataframe() # the 8 members, ordered
# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()] Shell
# sddb client: download every member .zarr into data/
sddb-cli download --collection geo-gse335702 --dest data/Licenses: 4 NCBI GEO policy, 4 CC BY 4.0
About the study
Pericyte K<sub>ATP</sub> channel hyperactivity redistributes cortical blood flow in a CADASIL mouse model.
Cerebral hemodynamic dysfunction is a key driver of unhealthy brain aging. Impaired microcirculatory reactivity leads to uneven perfusion, rendering deeper brain regions more vulnerable and thereby contributing to cognitive decline. Yet how capillaries contribute to these deficits remains poorly defined. Here we combined spatial transcriptomics with in vivo two-photon and three-photon imaging to measure layer-specific cerebral blood flow in control and small vessel disease model mice (CADASIL TgNotch3R169C). We found downregulation of ATP-synthesizing genes, indicating microvascular metabolic impairment that paralleled impaired pericyte bioenergetics. This energy deficit coincided with diminished tone in the arteriole-capillary transitional zone and reduced deep-layer perfusion. Complementary electrophysiology, ex vivo and in silico approaches, revealed that hyperactive K<sub>ATP</sub> channels in pericytes drive a redistribution of cerebral blood flow toward superficial cortical layers. This loss of spatial perfusion equalization, despite preserved global flow, contributed to deep-layer hypoperfusion, establishing a previously underrecognized but tractable vascular function disrupted in aging pathology.
Member datasets · 8
Mouse brain
GSM9817886
mouse brain 674 MB 217,363 bins bronze H&E NCBI GEO policy
TechnologyVisium HD
Organismmouse
Tissuebrain
Bins217,363
bronze H&E NCBI GEO policy
Mouse brain
GSM9817887
mouse brain cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1 1.1 GB 346,469 bins bronze H&E NCBI GEO policy
TechnologyVisium HD
Organismmouse
Tissuebrain
Conditioncerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1
Bins346,469
bronze H&E NCBI GEO policy
Mouse brain
GSM9817888
mouse brain cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1 700 MB 267,238 bins bronze H&E NCBI GEO policy
TechnologyVisium HD
Organismmouse
Tissuebrain
Conditioncerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1
Bins267,238
bronze H&E NCBI GEO policy
Mouse brain
GSM9817881
mouse brain 43.1 MB H&E
TechnologyVisium
Organismmouse
Tissuebrain
H&E
Mouse brain
GSM9817882
mouse brain cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1 29.7 MB 2,077 spots silver H&E
TechnologyVisium
Organismmouse
Tissuebrain
Conditioncerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1
Spots2,077
silver H&E
Mouse cerebral blood vessel
GSM9817883
mouse cerebral blood vessel 25.0 MB 831 spots silver H&E
TechnologyVisium
Organismmouse
Tissuecerebral blood vessel
Spots831
silver H&E
Mouse cerebral blood vessel
GSM9817884
mouse cerebral blood vessel cerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1 26.4 MB 632 spots silver H&E
TechnologyVisium
Organismmouse
Tissuecerebral blood vessel
Conditioncerebral arteriopathy, autosomal dominant, with subcortical infarcts and leukoencephalopathy, type 1
Spots632
silver H&E
Cite this collection
doi.org/10.1038/s44161-026-00844-0 ↗ BibTeX
@article{Jeffrey_2026,
title = {Pericyte KATP channel hyperactivity redistributes cortical blood flow in a CADASIL mouse model},
volume = {5},
ISSN = {2731-0590},
url = {http://dx.doi.org/10.1038/s44161-026-00844-0},
DOI = {10.1038/s44161-026-00844-0},
number = {8},
journal = {Nature Cardiovascular Research},
publisher = {Springer Science and Business Media LLC},
author = {Jeffrey, Danielle A. and Prince, Eric W. and Khakpour, Niloufar and Ferris, Hannah R. and Peters, Colin H. and Seedorf, Gregory and Romero, Phinea Z. and Bueno Guerrero, Mayra and Russell, Abigail N. and Glodoski, Katherine and Futia, Gregory L. and Bonney, Stephanie K. and Thornton, Michael A. and Gibson, Emily A. and Proenza, Catherine and Garcia, Anastacia M. and Tsoukias, Nikolaos M. and Dabertrand, Fabrice},
year = {2026},
month = Aug,
pages = {725–743}
}