Collections / Spatial transcriptomic analysis of human iPSC-derived hemanoids at day 16 and day 28

Spatial transcriptomic analysis of human iPSC-derived hemanoids at day 16 and day 28

Human induced pluripotent stem cell (iPSC)-derived hemanoids provide a three-dimensional model to study early hematopoietic development in vitro. To characterize the spatial organization and transcriptional dynamics of this system, we performed spatial transcriptomic profiling of FFPE hemanoids at day 16 and day 28 of differentiation using the 10x Genomics Visium Spatial Gene Expression platform (Visium v1 chemistry). This dataset captures the identification and spatial distribution of hematopoietic cells within the three-germ-layer-derived hemanoid environment.

Datasets2
Size~29.4 MB
AssayVisium
Samplehuman · hematopoietic system
KindTime course
Flag a data issue
www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE324601 ↗
Python · sddb client
from sddb import Catalog

cohort = Catalog().collection("geo-gse324601")
cohort.members        # the 2 datasets as a table

print(cohort.licenses())              # check the terms before you download

sdatas = cohort.to_dict()             # {uid: SpatialData}, cached locally
# or keep the .zarr files in a folder of your choice:
# sdatas = cohort.to_dict(dest="data/")
Python · lamindb

Needs pip install lamindb and network access to the Lamin database, which is limited during the alpha. If it fails, use the Client tab.

import lamindb as ln

db = ln.DB("scverse/spatialdata-db")
coll = db.Collection.get(key="geo-gse324601")
coll.ordered_artifacts.to_dataframe()   # the 2 members, ordered

# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()]
Shell
# sddb client: download every member .zarr into data/
sddb-cli download --collection geo-gse324601 --dest data/

Licenses: 2 NCBI GEO policy

Open in Vitessce
Visium
Human hematopoietic system
GSM9580061
human hematopoietic system 16.3 MB 1,024 spots silver H&E NCBI GEO policy
TechnologyVisium
Organismhuman
Tissuehematopoietic system
Size16.3 MB
Spots1,024
silver H&E NCBI GEO policy Open in Vitessce
Open in Vitessce
Visium
Human hematopoietic system
GSM9580062
human hematopoietic system 13.1 MB 1,354 spots silver H&E NCBI GEO policy
TechnologyVisium
Organismhuman
Tissuehematopoietic system
Size13.1 MB
Spots1,354
silver H&E NCBI GEO policy Open in Vitessce

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