Mouse barrel cortex
GSM9011928
mouse barrel cortex 84.4 MB 239,321 cells bronze NCBI GEO policy
TechnologyVizgen MERSCOPE
Organismmouse
Tissuebarrel cortex
Cells239,321
bronze NCBI GEO policy
Collections / geo-gse298314
geo-gse298314
Datasets4
Size~266 MB
AssayVizgen MERSCOPE
Samplemouse · barrel cortex
KindPublication
Download the whole collection
Python · sddb client
from sddb import Catalog
cohort = Catalog().collection("geo-gse298314")
cohort.members # the 4 datasets as a table
print(cohort.licenses()) # check the terms before you download
sdatas = cohort.to_dict() # {uid: SpatialData}, cached locally
# or keep the .zarr files in a folder of your choice:
# sdatas = cohort.to_dict(dest="data/") Python · lamindb
Needs pip install lamindb and network access to the Lamin database, which is limited during the alpha. If it fails, use the Client tab.
import lamindb as ln
db = ln.DB("scverse/spatialdata-db")
coll = db.Collection.get(key="geo-gse298314")
coll.ordered_artifacts.to_dataframe() # the 4 members, ordered
# download every member .zarr to the local cache:
paths = [a.cache() for a in coll.ordered_artifacts.all()] Shell
# sddb client: download every member .zarr into data/
sddb-cli download --collection geo-gse298314 --dest data/Licenses: 4 NCBI GEO policy
About the study
Microglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling.
Astrocytes and microglia are emerging key regulators of activity-dependent synapse remodeling that engulf and remove synapses in response to changes in neural activity. Yet, the degree to which these cells communicate to coordinate this process remains an open question. Here, we use whisker removal in postnatal mice to induce activity-dependent synapse removal in the barrel cortex. We show that astrocytes do not engulf synapses in this paradigm. Instead, astrocytes reduce contact with synapses prior to microglia-mediated synapse engulfment. We further show that the reduced astrocyte-synapse contact is dependent on the release of Wnts from microglia downstream of neuron-to-microglia fractalkine ligand-receptor (CX3CL1-CX3CR1) signaling. These results demonstrate an activity-dependent mechanism by which microglia instruct astrocyte-synapse interactions, providing a permissive environment for microglia to remove synapses. We further show that this mechanism is critical to remodel synapses in a changing sensory environment and that this signaling is upregulated in several disease contexts.
Member datasets · 4
Mouse barrel cortex
GSM9011925
mouse barrel cortex 57.0 MB 210,307 cells bronze NCBI GEO policy
TechnologyVizgen MERSCOPE
Organismmouse
Tissuebarrel cortex
Cells210,307
bronze NCBI GEO policy
Mouse barrel cortex
GSM9011926
mouse barrel cortex 61.5 MB 211,398 cells bronze NCBI GEO policy
TechnologyVizgen MERSCOPE
Organismmouse
Tissuebarrel cortex
Cells211,398
bronze NCBI GEO policy
Mouse barrel cortex
GSM9011927
mouse barrel cortex 62.8 MB 202,554 cells bronze NCBI GEO policy
TechnologyVizgen MERSCOPE
Organismmouse
Tissuebarrel cortex
Cells202,554
bronze NCBI GEO policy
Cite this collection
No linked publication found — please cite the data source.